Fluoride-activated photothermal system for promoting bacteria-infected wound healing.

Although photothermal therapy (PTT) employing nanozymes has shown excellent antibacterial potential, excessive heating generally harms host cells and hinders recovery. Herein, we report an innovative technique for acquiring the programmed temperature by managing the catalytic activity of nanozymes. The photothermal system of CeO2 + F- + TMB can obtain precise photothermal temperature by adjusting the concentration of fluoride ions under near-infrared irradiation. At the optimized photothermal temperature, the photothermal system affords fine photothermal antibacterial treatment with high-efficiency antibacterial effects against Staphylococcus aureus and Escherichia coli in vitro. In vivo wound healing experiments confirm that the system can effectively promote fibroblast proliferation, angiogenesis and collagen deposition with remarkable wound healing efficiency. This strategy offers a novel design concept for creating a new generation of PTT and opens the way for the creation of alternative antibiotics.

Multicomponent structural color membrane based on soft lithography array for high-sensitive Raman detection.

Inspired by ordered photonic crystals and structural color materials in nature, we successfully prepared hydroxypropyl cellulose (HPC) photonic films with ordered surface arrays by double-imprint soft lithography. Then we introduced another important material of the cellulose family, cellulose nanocrystals (CNC), which has liquid crystal nature and birefringent properties of the particles, into the system to realize the single-point shrinkage of the film array and the control of structural color. Through multi-component doping and concentration control, we further optimized the multi-scale structure of the materials, and obtained HPC/CNCs composite photonic films with excellent properties in color, stability and flexibility, whose elastic modulus and tensile properties are significantly higher than those of single-component. Further loading of SiO2@PDA enhances the color saturation and realizes the in-situ reduction of metal ions on the film surface. This plasma film can track a variety of substances with high sensitivity and long-term stability, showing potential application prospects in the field of surface-enhanced Raman scattering (SERS), which provides a potential possibility for chiral structures to be used in the field of biosensor detection and circularly polarized luminescence.

Engineering a Red Fluorescent Protein Chromophore for Visualization of RNA G-Quadruplexes.

Synthetic red fluorescent protein (RFP) chromophores have emerged as valuable tools for biological imaging and therapeutic applications, but their application in the visualization of endogenous RNA G-quadruplexes (G4s) in living cells has been rarely reported so far. Here, by integrating the group of the excellent G4 dye ThT, we modulate RFP chromophores to create a novel fluorescent probe DEBIT with red emission. DEBIT selectively recognizes the G4 structure with the advantage of strong binding affinity, high selectivity, and excellent photostability. Using DEBIT as a fluorescent indicator, the real-time monitoring of RNA G4 in biological systems can be achieved. In summary, our work expands the application of synthetic RFP chromophores and provides an essential dye category to the classical G4 probes.